Experiment 001B Extended — Multi-Run Per-Electrode Profiles

November 15–26, 2025 · Three Experimental Runs

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Overview

Motivation. This analysis addresses two key questions: (1) Do electrodes track together in a single connected substrate? (2) Do temperature fluctuations in outdoor environments cause the diurnal variations observed in saline controls? We extended the per-electrode analysis from Experiment 001B to Experiment 003's three runs. The hypothesis: one pool of saline should resemble one block of fungus—both represent potentially connected substrates where all electrodes sample the same electrical domain. Additionally, comparing outdoor (Run 2) vs temperature-controlled indoor (Run 3) conditions tests whether ambient temperature fluctuations and evaporation drive the "noon dip" patterns seen in previous saline controls.

Approach. For each run, we resample each electrode (a0–a3) at 5-minute intervals, plot per-day traces, and examine per-electrode patterns using the same methodology as Experiment 001B. ESPs analyzed: ESP06, ESP12, ESP15, ESP21.

Runs. Run 1: November 15-17, 2025 (cups configuration—electrodes in separate cups). Run 2: November 19-21, 2025 (bottle, outdoor—all electrodes in single pool, exposed to ambient temperature fluctuations). Run 3: November 21-26, 2025 (bottle, AC room—same single pool, temperature-controlled environment).

Results. Electrodes track together in the single-pool configurations (Runs 2 and 3), confirming that a connected substrate (whether saline or fungal) produces coherent electrode behavior. This supports the hypothesis that one pool of saline resembles one block of fungus in terms of electrical connectivity. Additionally, comparison of Run 2 (outdoor) vs Run 3 (AC room) demonstrates that temperature-controlled conditions dramatically reduce diurnal fluctuations, supporting the hypothesis that ambient temperature and evaporation drive the patterns observed in outdoor saline controls.

Use the panels below to switch between runs and ESPs, then scrub through the days with the slider.

Run 1: Cups Configuration

November 15-17, 2025

How to use. Choose an ESP from the menu, then scrub through the days with the slider. "Show all" restores all days so you can compare days side-by-side.

ESP06

Run 1: Cups Configuration

ESP06 hourly mean per electrode - Run 1
Hourly mean per electrode for ESP06 (Run 1: Cups Configuration).

15 Nov 2025

ElectrodeSamples
A02,680
A12,680
A22,680
A32,680
ESP06 per-electrode traces on 15 Nov 2025
ESP06 electrodes (a0–a3) captured on 15 Nov 2025. Each line represents an individual electrode sampled every few seconds, resampled here to 5-minute means.

16 Nov 2025

ElectrodeSamples
A041,261
A141,261
A241,261
A341,261
ESP06 per-electrode traces on 16 Nov 2025
ESP06 electrodes (a0–a3) captured on 16 Nov 2025. Each line represents an individual electrode sampled every few seconds, resampled here to 5-minute means.

17 Nov 2025

ElectrodeSamples
A037,380
A137,380
A237,380
A337,380
ESP06 per-electrode traces on 17 Nov 2025
ESP06 electrodes (a0–a3) captured on 17 Nov 2025. Each line represents an individual electrode sampled every few seconds, resampled here to 5-minute means.

Run 2: Bottle, Outdoor

November 19-21, 2025

How to use. Choose an ESP from the menu, then scrub through the days with the slider. "Show all" restores all days so you can compare days side-by-side.

ESP06

Run 2: Bottle, Outdoor

ESP06 hourly mean per electrode - Run 2
Hourly mean per electrode for ESP06 (Run 2: Bottle, Outdoor).

19 Nov 2025

ElectrodeSamples
A024,449
A124,449
A224,449
A324,449
ESP06 per-electrode traces on 19 Nov 2025
ESP06 electrodes (a0–a3) captured on 19 Nov 2025. Each line represents an individual electrode sampled every few seconds, resampled here to 5-minute means.

20 Nov 2025

ElectrodeSamples
A038,285
A138,285
A238,285
A338,285
ESP06 per-electrode traces on 20 Nov 2025
ESP06 electrodes (a0–a3) captured on 20 Nov 2025. Each line represents an individual electrode sampled every few seconds, resampled here to 5-minute means.

21 Nov 2025

ElectrodeSamples
A019,381
A119,381
A219,381
A319,381
ESP06 per-electrode traces on 21 Nov 2025
ESP06 electrodes (a0–a3) captured on 21 Nov 2025. Each line represents an individual electrode sampled every few seconds, resampled here to 5-minute means.

Run 3: Bottle, AC Room

November 21-26, 2025

How to use. Choose an ESP from the menu, then scrub through the days with the slider. "Show all" restores all days so you can compare days side-by-side.

ESP06

Run 3: Bottle, AC Room

ESP06 hourly mean per electrode - Run 3
Hourly mean per electrode for ESP06 (Run 3: Bottle, AC Room).

21 Nov 2025

ElectrodeSamples
A017,164
A117,164
A217,164
A317,164
ESP06 per-electrode traces on 21 Nov 2025
ESP06 electrodes (a0–a3) captured on 21 Nov 2025. Each line represents an individual electrode sampled every few seconds, resampled here to 5-minute means.

22 Nov 2025

ElectrodeSamples
A040,487
A140,487
A240,487
A340,487
ESP06 per-electrode traces on 22 Nov 2025
ESP06 electrodes (a0–a3) captured on 22 Nov 2025. Each line represents an individual electrode sampled every few seconds, resampled here to 5-minute means.

23 Nov 2025

ElectrodeSamples
A041,062
A141,062
A241,062
A341,062
ESP06 per-electrode traces on 23 Nov 2025
ESP06 electrodes (a0–a3) captured on 23 Nov 2025. Each line represents an individual electrode sampled every few seconds, resampled here to 5-minute means.

24 Nov 2025

ElectrodeSamples
A038,063
A138,063
A238,063
A338,063
ESP06 per-electrode traces on 24 Nov 2025
ESP06 electrodes (a0–a3) captured on 24 Nov 2025. Each line represents an individual electrode sampled every few seconds, resampled here to 5-minute means.

25 Nov 2025

ElectrodeSamples
A038,093
A138,093
A238,093
A338,093
ESP06 per-electrode traces on 25 Nov 2025
ESP06 electrodes (a0–a3) captured on 25 Nov 2025. Each line represents an individual electrode sampled every few seconds, resampled here to 5-minute means.

26 Nov 2025

ElectrodeSamples
A027,321
A127,321
A227,321
A327,321
ESP06 per-electrode traces on 26 Nov 2025
ESP06 electrodes (a0–a3) captured on 26 Nov 2025. Each line represents an individual electrode sampled every few seconds, resampled here to 5-minute means.

Temperature Fluctuation Analysis: Outdoor vs AC Room

Objective. Quantify the reduction in diurnal fluctuations when moving from outdoor (Run 2) to temperature-controlled indoor (Run 3) conditions. This addresses the "Temperature-Controlled Saline Repeat" future experiment proposed in the main analysis library.

To quantify the effect of temperature control on electrode fluctuations, we calculated the distribution of hourly mean values over 24 hours for both Run 2 (bottle, outdoor) and Run 3 (bottle, AC room). The metrics below measure how much the hourly means vary across the day:

Fluctuation Metrics Comparison

Metric Run 2 (Outdoor) Run 3 (AC Room) Reduction
Std of hourly means
Measures spread of hourly averages		 544.47 96.03 82.4%
Range of hourly means
Max - min across 24 hours		 1,521.38 395.90 74.0%
Coefficient of Variation
Normalized measure (std/mean)		 0.0623 0.0097 84.4%

Per-ESP Breakdown

ESP Run 2 Std Run 3 Std Reduction
ESP06 603.26 90.02 85.1%
ESP12 657.41 104.51 84.1%
ESP15 266.81 81.00 69.6%
ESP21 650.41 108.59 83.3%

Conclusion. Moving from outdoor (Run 2) to temperature-controlled indoor (Run 3) conditions results in a 74–84% reduction in diurnal fluctuations across all measured metrics. This provides strong quantitative evidence that ambient temperature fluctuations and evaporation drive the "noon dip" patterns observed in outdoor saline controls.

Implications. While the AC room conditions are not perfectly controlled (temperature still varies somewhat), the dramatic reduction in fluctuations suggests that in a fully temperature-controlled environment with constant temperature maintained throughout 24 hours, we would expect even less fluctuation. This validates the hypothesis that the diurnal patterns in outdoor saline controls are primarily environmental artifacts rather than biological signals.

Future work. A fully thermostated saline control with constant temperature would provide an even cleaner hardware baseline, but the current data already demonstrates that temperature control substantially reduces the fluctuations observed in outdoor conditions.